A student investigated the effect of pH on the time taken for amylase to break down starch.
She mixed amylase, starch and a buffer solution in a water bath at 35 °C. Every 30 seconds she put a drop of the mixture onto iodine solution on a spotting tile. She recorded the time when the iodine no longer turned blue-black.
Time for starch to be broken down: pH 5, 150 s; pH 6, 90 s; pH 7, 60 s; pH 8, 120 s; pH 9, 240 s.
pH
Time for starch to be broken down (s)
5
150
6
90
7
60
8
120
9
240
.1
The rate of reaction can be found with:
rate =time1000
Calculate the rate of reaction at pH 7.
[1 mark]
.2
Explain why the starch took much longer to break down at pH 9.
[2 marks]
.3
Suggest how the student could find the optimum pH of amylase more precisely.
[1 mark]
.4
Sampling every 30 seconds limits the accuracy of the times.
Explain why.
[1 mark]
6 marksFoundation and Higher
Spec and skills
Original practice question
Answer and supported method checks
White blood cells help to defend the body against pathogens.
.1
What are the molecules on the surface of a pathogen that white blood cells recognise as foreign?
[1 mark]
.2
Describe three ways white blood cells defend the body against pathogens.
[3 marks]
.3
A person has antibodies against the measles virus. These antibodies do not protect the person against the mumps virus.
Explain why.
[2 marks]
5 marksFoundation and Higher
Spec and skills
Original practice question
Answer and supported method checks
Heart muscle contracts all the time, throughout life.
.1
Explain why heart muscle cells contain many mitochondria.
[2 marks]
.2
Compare aerobic respiration with anaerobic respiration in muscle cells.
[3 marks]
4 marksFoundation
Spec and skills
Original practice question
Answer and supported method checks
Animal cells, plant cells and bacterial cells contain different structures.
.1
What is the function of mitochondria?
[1 mark]
.2
Name the structure in a plant cell that absorbs light for photosynthesis.
[1 mark]
.3
Which two structures are found in a bacterial cell? Tick two boxes.
[2 marks]
3 marksFoundation
Spec and skills
Original practice question
Answer and supported method checks
A student put some pondweed in a beaker of water under a lamp. She collected the oxygen given off in an upturned 25 cm³ measuring cylinder that was full of water at the start.
The measuring cylinder's scale starts at 0 cm³ at its closed end, which is now at the top. As oxygen collects at the top, it pushes the water down.
After 10 minutes, the boundary between the oxygen and the water was at the 4.0 cm³ mark.
.1
What volume of oxygen did the student collect?
[1 mark]
.2
Calculate the rate of oxygen production in cm³ per minute.
[1 mark]
.3
Another student counted the bubbles of oxygen instead.
Suggest why measuring the volume of gas gives more accurate results than counting bubbles.
