Higher tier students must complete Punnett squares and explain the outcomes. In genetic engineering, enzymes cut the required gene out, and the gene is inserted into a vector, usually a bacterial plasmid or a virus, which carries it into the target cells. Genes are put into plants or animals at an early stage so the characteristic develops. Benefits include crops resistant to disease; concerns include effects on wild populations and human health.
Write the parents' gametes.
Fill in the Punnett square.
State the ratio of phenotypes.
Watch out A 3:1 ratio of phenotypes is not the same as a 1:2:1 ratio of genotypes.
See it worked
Worked example
Describe how a gene is inserted into a bacterial cell.
Enzymes cut the gene out of the donor DNA.
The gene is inserted into a plasmid vector.
The plasmid is taken up by the bacterium, which then makes the protein.
Answer Enzymes cut out the gene, insert it into a plasmid, and the plasmid enters the bacterium
Your turn
Question 1Calculator allowed·2 marks
Two Tt pea plants are crossed. T (tall) is dominant. Out of 400 offspring, how many would you expect to be short?
Question 2Calculator allowed·1 mark
What is used as a vector in genetic engineering?
Question 3Calculator allowed·1 mark
A cross of Tt × Tt gives a phenotype ratio of:
In past papers
21 official questions in 14 papers since 2018. The latest: