GCSE Combined Science revision · Biology: homeostasis, inheritance and ecology

Higher: genetic crosses and engineering

Higher only

The idea

Higher tier students must complete Punnett squares and explain the outcomes. In genetic engineering, enzymes cut the required gene out, and the gene is inserted into a vector, usually a bacterial plasmid or a virus, which carries it into the target cells. Genes are put into plants or animals at an early stage so the characteristic develops. Benefits include crops resistant to disease; concerns include effects on wild populations and human health.

  1. Write the parents' gametes.
  2. Fill in the Punnett square.
  3. State the ratio of phenotypes.

Watch out A 3:1 ratio of phenotypes is not the same as a 1:2:1 ratio of genotypes.

See it worked

Worked example

Describe how a gene is inserted into a bacterial cell.

  1. Enzymes cut the gene out of the donor DNA.
  2. The gene is inserted into a plasmid vector.
  3. The plasmid is taken up by the bacterium, which then makes the protein.

Answer Enzymes cut out the gene, insert it into a plasmid, and the plasmid enters the bacterium

Your turn

Question 1Calculator allowed2 marks

Two Tt pea plants are crossed. T (tall) is dominant. Out of 400 offspring, how many would you expect to be short?

Question 2Calculator allowed1 mark

What is used as a vector in genetic engineering?

Choose one
Question 3Calculator allowed1 mark

A cross of Tt × Tt gives a phenotype ratio of:

Choose one

In past papers

21 official questions in 14 papers since 2018. The latest:

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Independent practice for AQA GCSE Combined Science: Trilogy (8464), not endorsed by AQA.

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