GCSE Combined Science revision · Biology: cells, organisation and disease

Microscopy and magnification

The idea

Electron microscopes have a much higher magnification and resolution than light microscopes, so they show sub-cellular structures such as ribosomes in detail. Magnification = size of image ÷ size of real object. Small sizes are measured in micrometres (µm): 1 mm = 1000 µm.

  1. Convert both sizes to the same unit.
  2. Use magnification = image size ÷ real size.
  3. Rearrange: real size = image size ÷ magnification.

Watch out Forgetting to convert units: 1 mm = 1000 µm, so a 5 mm image of a 50 µm cell is magnified 100 times, not 0.1 times.

See it worked

Worked example

A cell is 25 µm long. Its image in a drawing is 50 mm long. Calculate the magnification.

  1. 50 mm = 50 000 µm.
  2. Magnification = 50 000 ÷ 25 = 2000.

Answer × 2000

Your turn

Question 1Calculator allowed2 marks

An image of a cell is 12 mm wide. The magnification is × 400. What is the real width of the cell in micrometres?

Question 2Calculator allowed1 mark

Why can an electron microscope show ribosomes when a light microscope cannot?

Choose one
Question 3Calculator allowed2 marks

A mitochondrion is 2 µm long. A photograph shows it 30 mm long. What is the magnification?

In past papers

59 official questions in 41 papers since 2018. The latest:

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